Lacticaseibacillus rhamnosus LRH30, a type of lactic acid bacteria, can make special substances called exopolysaccharides.

These substances help to support our immune system and can be used as natural additives in food. Recent research focused on making the production of these substances more efficient.

Scientists used small bioreactors (like tiny factories) to test different conditions for growing bacteria.  They experimented with temperature and airflow to find the best settings for making more biomass (the actual bacterial substance) and exopolysaccharides.  They tested various amounts of airflow and temperatures for growing the bacteria.

The best conditions for making more biomass were found to be a temperature of 37. 01 degrees Celsius with an airflow of 0. 12 vvm. For making more exopolysaccharides, the ideal conditions were a cooler 20. 1 degrees Celsius with an airflow of 0. 18 vvm. When these conditions were used in small-scale tests, they produced about 10. 1 grams of biomass per liter and 520. 2 milligrams of exopolysaccharides per liter.

When the process was scaled up to larger 2-liter reactors, the biomass yield dropped by 18% to 8. 54 grams per liter.  However, the exopolysaccharides yield increased by 26% to 654. 6 milligrams per liter.  These results show that scaling up the process is more effective for making exopolysaccharides than for making biomass.

So, how did they know these substances were effective for supporting immune systems? They tested them on cells that act like immune cells in the body (called J774A. 1 murine macrophages). The results showed that both the bacteria and the exopolysaccharides had a mainly anti-inflammatory effect.

This research shows that it is possible to make natural food additives from bacteria.  By optimizing the conditions for growing the bacteria, it is possible to produce more of these substances efficiently.  This could lead to better and healthier food additives in the future.  However, it is important to note that more research is needed to fully understand the potential of these substances and to develop them for commercial use.

More research is needed to fully understand the potential of these substances in promoting health and to develop them for commercial use. It is also important to consider the environmental impact of scaling up the production process and to find ways to make it more sustainable.

Probing the Power of Bacteria for Better Food Additives

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