How Tiny Heat Waves Can Spot Proteins
Proteins play a crucial role in diseases, drugs, and research. Spotting them quickly and accurately is essential. A new method using microscale thermophoresis (MST) makes this possible.
How It Works
Aptamers: Scientists use two special pieces of DNA called aptamers that stick to the protein like magnets. These aptamers have a tiny matching sequence at their ends.
Fluorescent Marker: One aptamer is tagged with a fluorescent marker. When they meet the protein, they form a sandwich, bringing the matching sequences close together.
DNA Switch: This allows the sequences to zip together, creating a DNA switch.
Heat Wave: When scientists heat up the mix, the DNA switch opens up. This change can be measured, giving a signal that the protein is present.
Advantages
- Quick and Sensitive
- No Complicated Steps
- Used to Detect Proteins Like Thrombin and PDGF-BB
- Potential for Future Protein Detection
This method is a promising tool for medicine and research.
